- Methodology article
- Open Access
Strategies for improving approximate Bayesian computation tests for synchronous diversification
- Isaac Overcast^{1, 2}Email authorView ORCID ID profile,
- Justin C. Bagley^{3, 4} and
- Michael J. Hickerson^{1, 2}
https://doi.org/10.1186/s12862-017-1052-6
© The Author(s). 2017
Received: 26 April 2017
Accepted: 14 August 2017
Published: 24 August 2017
Abstract
Background
Estimating the variability in isolation times across co-distributed taxon pairs that may have experienced the same allopatric isolating mechanism is a core goal of comparative phylogeography. The use of hierarchical Approximate Bayesian Computation (ABC) and coalescent models to infer temporal dynamics of lineage co-diversification has been a contentious topic in recent years. Key issues that remain unresolved include the choice of an appropriate prior on the number of co-divergence events (Ψ), as well as the optimal strategies for data summarization.
Methods
Through simulation-based cross validation we explore the impact of the strategy for sorting summary statistics and the choice of prior on Ψ on the estimation of co-divergence variability. We also introduce a new setting (β) that can potentially improve estimation of Ψ by enforcing a minimal temporal difference between pulses of co-divergence. We apply this new method to three empirical datasets: one dataset each of co-distributed taxon pairs of Panamanian frogs and freshwater fishes, and a large set of Neotropical butterfly sister-taxon pairs.
Results
We demonstrate that the choice of prior on Ψ has little impact on inference, but that sorting summary statistics yields substantially more reliable estimates of co-divergence variability despite violations of assumptions about exchangeability. We find the implementation of β improves estimation of Ψ, with improvement being most dramatic given larger numbers of taxon pairs. We find equivocal support for synchronous co-divergence for both of the Panamanian groups, but we find considerable support for asynchronous divergence among the Neotropical butterflies.
Conclusions
Our simulation experiments demonstrate that using sorted summary statistics results in improved estimates of the variability in divergence times, whereas the choice of hyperprior on Ψ has negligible effect. Additionally, we demonstrate that estimating the number of pulses of co-divergence across co-distributed taxon-pairs is improved by applying a flexible buffering regime over divergence times. This improves the correlation between Ψ and the true variability in isolation times and allows for more meaningful interpretation of this hyperparameter. This will allow for more accurate identification of the number of temporally distinct pulses of co-divergence that generated the diversification pattern of a given regional assemblage of sister-taxon-pairs.
Keywords
- Approximate Bayesian computation (ABC)
- Comparative phylogeography
- Divergence times
- Synchronous diversification
Background
Over the last three decades, comparative phylogeographic studies have used population-level genetic data from regional biotic assemblages to investigate how earth history dynamics have contributed to regional patterns of biodiversity and community assembly [1–5]. The implementation of hierarchical Approximate Bayesian Computation [6–9] has become a key statistical approach in comparative phylogeography for using coalescent models to test hypotheses of vicariance and dispersal [10], synchronous isolation [11], and models of co-expansion [12, 13] and has subsequently been adopted in a diversity of fields including neurobiology and astronomy [8, 9]. Other statistical approaches have been recently developed for the analysis of comparative phylogeography under unified multi-species models [14], and hABC models using random-forest classification has been recently expanded to accommodate reduced-genome SNP data [4, 14, 15]. However, hABC was initially developed for the purpose of quantifying patterns of co-isolation across taxon-pairs [12] based on datasets typically consisting of animal mtDNA sequences.
In recent years, there has been some disagreement [16–18] concerning two key details of how hABC is implemented in the MTML-msBayes software pipeline [7]. Leaving aside the disagreements about careful choice of the prior distribution of divergence times, Pr(τ), and its impact on the finite sampling of hyperparameter space when implementing hABC [17], two other contested issues are how best to sample from the prior on the number of co-divergence events (Ψ) and whether to sort the summary statistic vector prior to the rejection step. For the former, it has been argued that implementing a Dirichlet-process prior (DPP) over Ψ reduces bias that leads to incorrect inference of synchronous divergence given a discrete uniform prior over Ψ [19]. For the latter, an argument has been made that sorting the summary statistic vector by ascending values of average pairwise divergence (π_{b}), in order to improve computational efficiency and accuracy in estimating the dispersion index of divergence times (Ω = Var(τ)/E(τ)), introduces bias favoring overestimation of the degree of synchronicity in divergence times [19]. Furthermore, it has been argued that using the sorting option leads to biased results and should be avoided because it treats species-specific elements within any one of the summary statistic classes as exchangeable across taxon-pair samples, irrespective of differences across taxa with regards to sequence length, number of sequences, DNA substitution model, as well as differences in mutation rates, and ploidies [19]. However, most of the summary statistic classes have equal expectation across numbers of individuals and sequence length (they are largely scaled per base pair) yet will have unequal variances. Furthermore, if there are differences in mutation rates, substitution models, or ploidies (which are all accommodated in the simulation step), this will be reflected consistently across simulations and the associated sorted vectors accordingly. While this sorting violates some of these aforementioned assumptions, it is likely that hABC estimation is robust to these deviations in most if not all single locus mtDNA datasets. In this case, violations of exchangability by sorting the vector are outweighed by information gained about the distribution of divergence times, similar to how the rank abundance curve extracts information about species abundance distributions in community ecology. As is common in models of population genetics, minor violations of some assumptions can lead to improved inference, and in this case the sorted vector can result in a greater potential correlation between the variability in divergence times and the distribution of summary statistic values within each summary statistic class of the sorted vector. Luckily one can always use simulation experiments to compare the efficacy of alternative summary statistic options by quantifying differences in accuracy and bias in posterior estimates given identical datasets simulated under known parameter values.
Beyond disagreements over these options, the far more fundamental factor underlying the difficulty in estimating the Ψ hyperparameter is the inherent lack of correlation between Ψ and the actual variability in divergence times. For example, if many distinct divergence events are temporally clustered in time, the correspondingly high Ψ value will have little relevance to the overall level of high synchronicity of divergences in a given assemblage. At the other extreme, Ψ = 2 could involve two co-divergence pulses that were 1 million years apart, corresponding to an overall high level of temporal discordance in the assemblage as manifested by high variability in pairwise divergences.
To improve upon estimation of Ψ, we introduce and demonstrate the implementation of a user-defined buffer, β, to enforce a minimum time between any two co-divergence pulses [4, 14, 15]. Appropriate values of β should increase the correlation between Ψ and the true variability in divergence times, thereby increasing the utility of Ψ for meaningful interpretation in the context of answering biogeographic questions at Pleistocene and Pliocene time scales. For example, if co-divergence events are hypothesized in the context of Quaternary glaciation cycles, one may be interested in testing whether a temperate community assemblage became significantly fractured during any one of the late Pleistocene glacial maxima or minima. In this case, one might consider using β values equivalent to 25,000 years such that divergences occurring within temporal windows of 50,000 years would be considered effectively part of the same co-divergence pulse.
In this paper, we use simulation-based cross-validation to evaluate how different β values impact the estimation of Ψ and Ω. We also implement a simulation-based two-by-two experiment to compare Ψ and Ω estimators under contrasting options for sorting the summary statistic vector, as well as contrasting the use of discrete uniform and DPP prior for Ψ. Finally, using what we learned from these simulation experiments, we reanalyze three sets of empirical data from assemblages representing a range of life-histories, data configurations, and sample sizes, such as are routinely analyzed in mitochondrial-based comparative phylogeographic studies.
Methods
Implementing buffered divergence times
Under the msBayes model, the number of co-divergence times is parameterized by Ψ under a discrete uniform prior. The value of Ψ can range from 1 to Y, where Y equals the number of taxon-pairs in question. A Ψ value of 1 signifies simultaneous divergence of all taxa, whereas Ψ = Y indicates fully asynchronous divergence. Given any history of more than one divergence time (Ψ > 1), any subset of the divergence times within the vector {τ_{0} , …, τ_{Ψ}} can be tightly clustered in time, making this parameter difficult to meaningfully interpret with respect to understanding the distribution of co-divergence pulses. In other words, this original parameterization of Ψ results in a poor correlation with the actual variability in divergence times and the summary statistics underlying this variability (π_{b} and π_{net}; [20]) and is a poor indicator for how many pulses of co-divergence there might actually have been. Although overall variability in τ is well quantified by Ω, the dispersion index of divergence times (Var(τ)/E(τ)), having good estimates of Ψ can be important for testing more detailed biogeographic hypotheses.
A more desirable property for Ψ would be for it to better reflect the effective number of divergence pulses. To this end, we implemented a modification to the MTML-msBayes [7] algorithm for sampling divergence times (τ) between pairs of sister taxa. As in the original implementation, 1 to Ψ different divergence times are randomly sampled from a prior distribution for τ. However, during the sampling process we incorporate a condition such that any two pulses of co-divergence within the vector {τ_{0} , …, τ_{Ψ}} of divergence times are separated by at least β units of time, effectively generating a buffer around divergence times of width 2*β. The process of randomly drawing each of the divergence times from the prior given each hyperprior draw of Ψ continues until all taxon-pairs are assigned divergence times conditional that all Ψ times are at least β coalescent time units from each other. Due to the dynamic between β and the uniform prior for τ, the upper limit of the discrete uniform prior for Ψ may be truncated since this limit is determined by τ / 2β. For example, if τ_{max} = 0.75 and β is set to 0.1, τ_{max} / 2β = 3.75 and therefore the maximum possible value of Ψ_{max} will be 3.
Simulation experiments assessing impact of β
We investigated the impact of varying β values on estimation of Ψ and Ω under a range of data configurations. To maintain direct comparison with previous simulation experiments of MTML-msBayes conducted by [21], we included a data configuration consistent with 18 sister-taxon-pairs of parasitoid wasps codistributed across the Iberian and Balkan regions. We also included two smaller data configurations of co-distributed Panamanian frogs (n = 4 taxon-pairs) and fishes (n = 3 taxon-pairs) (Bagley et al. in review). These data configurations reflect numbers of taxon-pairs, numbers of samples per taxon, and numbers of base pairs per locus commonly evaluated in single locus comparative phylogeographic studies [22–24]. In all three cases, we used simulation experiments to explore the bias and accuracy in estimates of Ψ and Ω given four different β values (0.0, 0.01, 0.05, and 0.1), using leave-one-out cross-validation [25]. For each β value and data configuration, we generated a reference table of 3 × 10^{6} random draws from the hyperprior. We then generated 100 PODS under the same β value and data configuration to compare the true values and mode estimates of Ψ and Ω. We quantified the impact of β on these estimates by calculating root mean squared error (RMSE) rates in aggregate, as well as RMSE as a function of the true parameter values.
Simulation experiments assessing summary statistic sorting options and hyperprior on Ψ
It has been suggested that the default option of resorting the summary statistics within the vector D by the magnitude of π_{b}, as described in [7], should be avoided for most empirical datasets [19] due to the possible lack of exchangeability of summary statistic classes across taxon-pairs potentially biasing towards the inference of synchronous co-divergence. Additionally, it has been suggested that the discrete uniform prior on Ψ also biases toward inference of synchronous co-divergence [19]. To directly compare the ability to estimate Ψ and Ω given these different decisions, we conducted a two-by-two experiment including both sorting strategy and the two hyperpriors for Ψ: the DPP as described by [19], and the discrete uniform hyperprior first introduced by [12]. We accomplished this by porting the implementation of the DPP from DPP-msbayes [19] into MTML-msBayes [7]. The new implementation along with all iPython notebook code for reproducing simulations and analyses are available on GitHub (https://github.com/Hickerlab/msBayes).
To increase comparability of the results for each hyperprior for Ψ, the sorted and unsorted reference tables of 3 × 10^{6} draws were generated from identically sampled random draws from the hyperprior with the only difference being the sorting strategy for D. This was done for all four combinations of sorting strategies and alternative hyperpriors for Ψ (DPP and uniform) to generate four different combinations of hABC options hereafter referred to as sorted uniform (U_{S}), sorted DPP (DPP_{S}), unsorted uniform (U_{U}), and unsorted DPP (DPP_{U}). For each estimate given an associated POD, we retained 1000 posterior samples for each hABC posterior estimate under each of the four reference tables associated with the four different hABC settings (i.e. U_{S}, DPP_{S}, U_{U}, and DPP_{U}). We used non-linear neural network regression during the post-acceptance rejection adjustment for Ψ [26], and local linear regression for Ω [27]. For this simulation experiment, we used the same three data configurations as above (3, 4, and 18 taxon-pairs). As above, we quantified error by calculating aggregate RMSE, as well as RMSE as a function of the true parameter values.
Applications to empirical data: Neotropical butterflies, frogs, and fishes
To explore the effects of various magnitudes of β on estimation of Ψ and Ω for real data, we reanalyzed three empirical datasets. This included data from Bagley et al. (in review) that tested variation in diversification times among population-pairs of Panamanian frogs (n = 4 taxon-pairs) and freshwater fishes (n = 3 taxon-pairs) using one mitochondrial sequence from each of 10 to 28 individuals per taxon. We also reanalyzed a large dataset of 116 sister species-pairs of Neotropical butterflies consisting of an average of 3.4 mitochondrial cytochrome c oxidase subunit I sequences per taxon [28]. Guided by our simulation investigation, we adopted a uniform prior on Ψ with order-independent sorting of the summary statistic vector and report results based on β = 0.0, 0.05, 0.01, and 0.1 (See Additional file 1 for complete details of empirical analysis methods).
To test the goodness of fit of our model, we used prior and posterior predictive simulations [29] implemented in the abc R package. For the the prior predictive goodness of fit, we plot the first two principal components of the observed and accepted simulated summary statistics given the approximate posterior distribution. For the posterior predictive goodness of fit test, we tested whether the observed summary statistics fell within the distribution of summary statistics that were calculated from re-simulated data given the approximate posterior distribution of Ψ.
Results
Impact of β on estimation of Ψ and Ω
RMSE on estimates of Ψ and Ω sampling the prior and PODS with varying β
β | 18 taxon-pairs U_{S} Ψ | 18 taxon-pairs U_{S} Ω | 18 taxon-pairs U_{U} Ψ | 18 taxon-pairs U_{U} Ω | 4 taxon-pairs U_{S} Ψ | 4 taxon-pairs U_{S} Ω | 3 taxon-pairs U_{S} Ψ | 3 taxon-pairs U_{S} Ω |
---|---|---|---|---|---|---|---|---|
0 | 4.443 | 0.0314 | 5.0695 | 0.064 | 0.866 | 0.1347 | 0.7874 | 0.0895 |
0.01 | 4.4531 | 0.0273 | 5.2278 | 0.0761 | 0.911 | 0.102 | 0.8062 | 0.0959 |
0.05 | 1.5264 | 0.0276 | 1.8055 | 0.0507 | 0.911 | 0.146 | 0.8 | 0.1048 |
0.1 | 0.6164 | 0.0298 | 0.8646 | 0.0392 | 0.9747 | 0.1317 | 0.7681 | 0.0909 |
Impact of summary statistic ranking and hyperprior of Ψ on the estimation of Ω and Ψ
RMSE on Ψ and Ω varying the prior on Ψ and the sorting method
18 taxon-pairs Ψ | 18 taxon-pairs Ω | 4 taxon-pairs Ψ | 4 taxon-pairs Ω | 3 taxon-pairs Ψ | 3 taxon-pairs Ω | |
---|---|---|---|---|---|---|
U_{S} | 4.6079; 4.141 | 0.0298; 0.0295 | 0.9695; 0.974 | 0.1052; 0.0952 | 0.8000; 0.8069 | 0.0850; 0.087 |
U_{U} | 5.1069; 4.892 | 0.0867; 0.0856 | 0.9592; 1.038 | 0.1242; 0.1139 | 0.8367; 0.7625 | 0.0949; 0.091 |
DPP_{S} | 5.339; 5.317 | 0.0286; 0.0303 | 1.0149; 0.698 | 0.1158; 0.1073 | 0.8246; 0.7937 | 0.0720; 0.0845 |
DPP_{U} | 5.8853; 5.058 | 0.0868; 0.0854 | 0.9; 0.92 | 0.1262; 0.1245 | 0.8124; 0.7454 | 0.0954; 0.092 |
Unlike the error dynamics associated with DPP_{S} and DPP_{U}, use of U_{S} and U_{U}, under all three data configurations (3, 4, and 18 taxon-pairs) resulted in estimation error that was more evenly distributed across all simulated true Ψ values regardless of sorting method (Fig. 3). Likewise, the RMSE on the estimation of Ω was generally low and unbiased across all true Ω values when using the sorting method (U_{S}). On the other hand, with U_{U} there was an increasing bias in RMSE with decreasing true values of Ω (Fig. 4). This pattern was similar across all data configurations, but was most pronounced with the 18 taxon-pair configuration.
Application to empirical datasets
Mode estimates of Ψ and Ω for empirical datasets under the most likely model, with and without buffering
β | β_{years} | Ψ_{mode} | E[τ] | Ω_{mode} | BF(Ψ = 1,Ψ > 1) | |
---|---|---|---|---|---|---|
Frogs | 0 | N/A | 1 | 0.106 | 0.0036 | 1 |
0.05 | 8.0 × 10^{4} | 1 | 0.486 | 0.010 | 1.80 | |
Fishes | 0 | N/A | 1 | 0.054 | 0.0017 | 1 |
0.05 | 8.9 × 10^{4} | 1 | 0.198 | 0.003 | 1.41 | |
Butterfly (Full) | 0 | N/A | 15 | 0.378 | 0.108 | ~0 |
0.05 | 3.2 × 10^{4} | 5 | 0.389 | 0.113 | ~0 |
For the reanalyisis of the 116 taxon-pairs of Neotropical butterflies, estimates of Ω and Ψ both suggest that asynchronous divergence was prevalent within butterflies across the Neotropics and, as predicted, the implementation of β resulted in an estimate of fewer co-divergence pulses than when β was set to 0. Similar to the smaller datasets, the mode and 95% HPD of Ω were not significantly different across buffering regimes (Mode estimate of Ω: β(0) = 0.11; β(0.01) = 0.12; β(0.05) = 0.11; β(0.1) = 0.12). However, increasing values of β had a strong impact on estimates of Ψ. Though (Pr(Ψ > 1) ≈ 1) held true across all buffering regimes, mode estimates varied considerably (Mode estimate of Ψ: β(0) = 15; β(0.01) = 24; β(0.05) = 5; β(0.1) = 5). This finding is in agreement with the divergence time estimates of Garzón-Orduña et al. [25], who obtained a wide range of divergence times without accounting for differences in population divergences and gene divergence predicted under the standard coalescent.
Goodness-of-fit tests based on the approximate posterior distribution for all three empirical datasets showed that, for all β values, the observed data fell within the cloud of simulated points in the PC plots (Additional file 1: Figs. S17, S18 and S19). Similarly, results of our posterior predictive simulations indicated that the observed summary statistics were not significantly different from the simulated summary statistics (p > 0.05 for all values of β), indicating a good fit of our model. Although the invariance of divergence that is assumed within each of the Ψ divergence pulses in our model using β is a simplified approximation, the prior and posterior predictive checks we implemented both show an acceptable goodness of fit demonstrating that our inferential model can generate all four datasets, a fundamental step for Approximate Bayesian Computation [29, 31, 32].
Discussion
Buffering improves estimation of co-divergence events
The dynamics between chosen β and τ _{max} values are important, as these constrain the sampling space of τ as well as the maximum value of Ψ, yet these dynamics also depend on the number of taxon-pairs analyzed. With few taxon-pairs, even relatively high values of β may not reduce the maximum value of Ψ (Ψ_{max}). However, as the number of taxon-pairs increases, increasing the value of β will reduce Ψ_{max} proportional to the number of taxon-pairs (Y) and τ_{max}. For example, the β values that we explored had little impact on Ψ_{max} for the 3 and 4 taxon-pairs data configurations. However, in the 18 taxon-pairs case, increasing values of β proportionally decreased Ψ_{max} (β = 0.05, Ψ_{max} = 7; β = 0.1, Ψ_{max} = 3). It is important to note that if τ_{max} / β < Y then Ψ_{max} < Y, which precludes the possibility of inferring fully idiosyncratic divergence. We recommend that researchers carefully consider β values that reflect a meaningful time scale of biogeographic interest in the context of co-demographic histories, and likewise consider τ _{max} values that are plausible with respect to observed gene-tree divergence times [17]. Obviously, choosing β = τ _{max} / 2 would not yield meaningful results, whereas a β value corresponding to 10,000–50,000 years would be within the level of resolution of interest for many biogeographic questions. Additionally, as with all comparative phylogeographic analysis, care must be taken in properly scaling time across study organism to account for variation in mutation rates, generation times and/or ploidy. The msBayes pipeline includes such a scaling factor per taxon per locus, to ensure meaningful inference of co-divergence within any given buffering regime.
An important simplification of our β implementation is that co-divergence times within a pulse are identical. While this is admittedly unrealistic, it is best to view adding a chosen β value to the model as a useful approximation that captures the overall pattern in the number of meaningful co-divergence pulses. One possible improvement to explore would be to allow each taxon-pair assigned to a particular τ, given a chosen β, to draw its own τ value from a normal distribution centered around τ with a standard deviation of 2β. This would maintain the integrity of the buffer as these taxon-pairs could still be considered to be “co-diverging”, but would also allow divergence times for each taxon-pair to vary in a way that could capture differences in timing due to stochastic and/or ecologically deterministic factors. A related complementary method could be developed by using the Poisson distribution to construct a test for overdispersion or clumpiness, with the null being a random distribution of divergence events across the prior on divergence times [33].
Impact of hyperprior for Ψ
Although the motivation for the DPP is to allow researchers to distribute prior probability across divergence models conditional on the number of possible models per Ψ value [19], thereby reducing bias against inference of multiple co-divergence events, we find choice of the prior has little impact on the estimation of Ψ. Here we show that, given a finite and reasonable number of samples from the prior, the concave shape of the plot of RMSE estimates on Ψ across PODS for the DPP does reflect the preponderance of weight placed on the prior for values of Ψ(Fig. 3). By contrast the RMSE of the uniform estimates are more evenly distributed, reflecting the reduced constraint and the increased ability of the uniform prior to accurately infer a greater range of true Ψ values. While these sampling dynamics make the DPP less prone to incorrectly reject truly asynchronous divergence histories if basing such tests on the hyperparameter Ψ [19], this comes with the cost of reducing power to infer histories of true synchronous divergence. Moreover, other factors explored here and elsewhere clearly demonstrate Ω to be a far superior metric for this test if one implements the sorting option [17, 21].
Application to empirical data
The effects of the choice of hyperprior for Ψ, implementation of β, and sorting of summary statistic elements were all reduced in the smaller datasets of 3 and 4 taxon-pairs, and as predicted, they did not change the outcome for the corresponding empirical frog and fish datasets. Inference from the larger butterfly dataset of 116 taxon-pairs was somewhat sensitive to these choices. Our estimate of Ω = 0.113 is consistent with an estimate of Ψ = 5 co-divergence pulses given β = 0.05. The prior and posterior predictive checks we implemented both show an acceptable goodness of fit, demonstrating that our inferential model can generate all three datasets, a fundamental step for hABC analysis [29, 33].
Conclusions
We have demonstrated that estimating the number of pulses of co-divergence across co-distributed taxon-pairs is improved by applying a flexible buffering regime over divergence times. This achieves better statistical properties by increasing correlation between the number of co-divergence pulses and the overall magnitude of variability in divergence times. This will allow for more accurate identification of the number of temporally distinct pulses of co-divergence that generated the diversification pattern of a given regional assemblage of sister-taxon-pairs. Additionally, we show that the choice of hyperprior on Ψ is not as important as the choice of whether to sort the summary statistics vector, as our simulation experiments clearly show that using the sorting strategy results in drastically improved estimates of the overall variability in divergence. Although the assumptions of exchangeability across units within this vector may not be met for many mtDNA datasets, as with many cases in population genetics and phylogenetics, violation of such assumptions can be outweighed by the improved accuracy gained from using a model with reduced complexity. We find the differences in bias and accuracy across all parameterizations become negligible given datasets composed of smaller numbers of taxon-pairs. Further, we find summary statistics are more tightly correlated with parameters of interest for larger numbers of taxon-pairs. An analysis specifically targeting this question would illuminate this dynamic, yet in general it is expected that estimation of variability in parameter values will improve with increasing numbers of taxa, as demonstrated in [14]As with any application of ABC in population genetics and phylogeography [34], we recommend the standard practice of using simulation experiments to test for robustness, and the use of goodness-of-fit tests to explore various summary statistic options, settings, and model hyperpriors in the context of one’s data and the computational resources at hand [35, 36].
Declarations
Acknowledgements
Funding was provided by grants from FAPESP (BIOTA, 2013/50297-0 to MJH and AC Carnaval), NASA through the Dimensions of Biodiversity Program (DOB 1343578) and the National Science Foundation (DEB-1253710 to MJH), and the CNPq Ciência Sem Fronteiras program (Processo 314724/2014-1 to JCB and F. Langeani Neto). This work would not have been possible without help from the City University of New York High Performance Computing Center, with support from the National Science Foundation (CNS-0855217 and CNS- 0958379), and the Brigham Young University Fulton Supercomputing Lab.
Availability of data and materials
All source code and the iPython notebooks for reproducing all analyses will be posted and freely available prior to publication.
Project home page: https://github.com/Hickerlab/msBayes
Archived version: <repository milestone will be updated prior to publication>
Operating systems: Mac/Linux
Programming languages: Perl, C, R, Python
License: GNU GPL
Authors’ contributions
IO and MH conceived and designed the study. IO implemented the code and the simulation analyses. IO and JCB executed the empirical analyses. IO and MH led the writing of the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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Authors’ Affiliations
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