Fig. 4

Genetic context of candidate gene examples. (a-h) Arrows designate identified orthologous TSSs. The transcription fold change (as in Fig. 3) is noted above the arrow. Arrow shade corresponds to the condition in which the TSS was upregulated: branchless (green) or true-branching (red). Distance to the next upstream and downstream 5′ ORF is noted to the left and right of the arrow respectively. ORFs for which the TSS was observed are shaded according to the condition where the TSS was upregulated: branchless (green) or true-branching (red). Neighbouring ORFs are coloured in grey. The genomic context is accompanied by a reaction norm where the phenotype (y-axis) is the TSS transcription level of F. muscicola (full circles) and F. thermalis (empty circles) in the different environments that induce the branchless and true-branching phenotypes (x-axis). Gene identifier of hypothetical ORFs: c) WP_009456078.1 (the amino acid sequence is identical between the two Fischerella species) d) F. muscicola: WP_016867660.1, F. thermalis: WP_016870793.1; e) F. muscicola: WP_016869437.1, F. thermalis: WP_009455441.1; f) F. muscicola: WP_016867107.1, F. thermalis: WP_009460349.1; g) F. muscicola: WP_016865184.1, F. thermalis: WP_009456028.1; h) F. muscicola: WP_016867004.1, F. thermalis: WP_016871273.1