Table 2 List of primers used for long-range PCR across all ABCB1 introns
From: Parallel loss of introns in the ABCB1 gene in angiosperms
Primers | Sequence | Annealing temp. (°C) | Species in which primers were successfully used |
|---|---|---|---|
Forward | |||
 ABCB1_F5 | GACCTCTTCCGCTTCGCCGACG | 61 | Zoysia |
 ABCB1_F1 | CGCTTCTTYGCSGABCTBGTSGACTC | 61 | Phyllostachys, Typha, Ecdieocolea, Mayaca, Paspalum, Streptochaeta |
Reverse | |||
 ABCB1_R4 | CCGTGATCTTGCGCTCCGCGTTG | 61 | Zoysia |
 ABCB1_R1 | GCGTASGASGCGTASAGSAGGAACTG | 61 | Phyllostachys, Typha, Ecdieocolea, Paspalum, Streptochaeta |
 ABCB1_R22 | GACACCATCAGCACCATGAA | 61 | Mayaca |