Table 1 Primer sequences
First-stage PCR specific primers | |
5S-F | 5’-TTTCCCAGTCACGACGTTATGCACCGGATCCCATCAGA-3′ |
5S-R | 5’-TAATACGACTCACTATAGGGAGTGCTGGTATGATCGCACC-3′ |
ITS-F | 5’-TTTCCCAGTCACGACGTTTCCTCCGCTTATTGATATGC-3′ |
ITS-R | 5’-TAATACGACTCACTATAGGGTCGTAACAAGGTTCCCGTAGGTG-3′ |
Second-stage PCR primers [19] | |
MIDх-454-F | 5’-GCCTCCCTCGCGCCATCAG-MIDx-TTTCCCAGTCACGACGTT-3’ |
MIDх-454-R | 5’-GCCTTGCCAGCCCGCTCAG-MIDx-TAATACGACTCACTATAGGG-3’ |