Table 2 Two-way ANCOVA results of cellular immune parameter from brooding vs. non-brooding females: Significant p values (p < 0.05) are marked in bold letters. Results from Tukey HSD posthoc tests can be found in Additional file 2: Table S2
| Â | Blood | Spleen | Head Kidney | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Df | SS | MS | F value | Pr(>F) | SS | MS | F value | Pr(>F) | SS | MS | F value | Pr(>F) |
Lymphocyte/Monocyte | |||||||||||||
 Treatment | 1 | 0.017 | 0.017 | 0.89 | 0.378 | 0.70 | 0.70 | 2.11 | 0.190 | 4.30 | 4.30 | 5.62 | 0.050 |
 Challenge | 1 | 0.008 | 0.008 | 0.43 | 0.531 | 0.07 | 0.07 | 0.20 | 0.667 | 0.07 | 0.07 | 0.09 | 0.775 |
 Condition factor | 1 | 0.018 | 0.018 | 0.94 | 0.364 | 0.01 | 0.01 | 0.03 | 0.861 | 0.10 | 0.10 | 0.13 | 0.733 |
 Treatment*Challenge | 1 | 0.006 | 0.006 | 0.34 | 0.580 | 0.07 | 0.07 | 0.21 | 0.660 | 0.02 | 0.02 | 0.02 | 0.887 |
 Residuals | 7 | 0.132 | 0.019 |  |  | 2.32 | 0.33 |  |  | 5.37 | 0.77 |  |  |
Active/Inactive Cells | |||||||||||||
 Treatment | 1 | 0.000 | 0.000 | 3.32 | 0.1113 | 0.51 | 0.51 | 0.54 | 0.488 | 0.00 | 0.00 | 7.29 | 0.031 |
 Challenge | 1 | 0.000 | 0.000 | 0.00 | 0.9643 | 0.23 | 0.23 | 0.24 | 0.638 | 0.00 | 0.00 | 0.08 | 0.783 |
 Condition factor | 1 | 0.000 | 0.000 | 0.13 | 0.7311 | 1.10 | 1.10 | 1.17 | 0.316 | 0.00 | 0.00 | 5.84 | 0.046 |
 Treatment*Challenge | 1 | 0.000 | 0.000 | 0.01 | 0.9433 | 0.05 | 0.04 | 0.05 | 0.834 | 0.00 | 0.00 | 0.40 | 0.546 |
 Residuals | 7 | 0.000 | 0.000 |  |  | 6.63 | 0.95 |  |  | 0.00 | 0.00 |  |  |