BMC Ecology and Evolution

Table 4 Two-way ANCOVA results of cellular immune parameter from males, reproducing and non-reproducing females: Significant p values (p < 0.05) are marked in bold letters. Results from Tukey HSD posthoc tests can be found in Additional file 4: Table S4

From: Parental investment matters for maternal and offspring immune defense in the mouthbrooding cichlid Astatotilapia burtoni

		Blood				Spleen				Head Kidney
	Df	SS	MS	F value	Pr(>F)	SS	MS	F value	Pr(>F)	SS	MS	F value	Pr(>F)
Lymphocyte/Monocyte
Treatment	2	14.50	7.25	12.25	<0.01	1.85	0.92	2.71	0.093	1.73	0.87	1.79	0.194
Challenge	1	0.60	0.60	1.01	0.33	0.01	0.01	0.03	0.859	0.14	0.14	0.29	0.594
Condition factor	1	1.06	1.06	1.79	0.20	0.40	0.40	1.17	0.295	0.12	0.12	0.24	0.629
Treatment*Challenge	1	0.77	0.77	1.30	0.27	0.06	0.06	0.17	0.681	0.39	0.39	0.81	0.378
Residuals	19	11.24	0.59			6.12	0.34			9.17	0.48
Active/Inactive Cells
Treatment	2	2.08	1.04	2.11	0.15	13.05	6.53	1.82	0.188	1.48	0.74	4.65	0.023
Challenge	1	0.73	0.73	1.47	0.24	10.32	10.32	2.87	0.106	0.00	0.00	0.02	0.887
Condition factor	1	1.23	1.23	2.50	0.13	4.62	4.62	1.29	0.270	0.48	0.48	2.99	0.100
Treatment*Challenge	1	0.37	0.37	0.75	0.40	1.12	1.12	0.31	0.582	0.05	0.05	0.33	0.570
Residuals	19	8.38	0.49			71.78	3.59			3.02	0.16

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