Fig. 2

Retinal radial sections of the primarily diurnal Tomodon dorsatus (a, c, e) and the primarily nocturnal Sibynomorphus neuwiedi (b, d, f), labeled with anti-opsin antibodies and agglutinins. a outer segments of double cones (*) and large single cones and b, outer segments of large singles cones, labeled by the antibody AB5405, against L/M cones, revealed in red by CY3. c and d, outer segments of small single cones, labeled by the antibody AB5407, against UV/VS cones, revealed in red by CY3. e and f, double labeling by the agglutinins PNA and WGA: outer segments of cones labeled by PNA, reveled in green by FITC, and the outer segments of small single cones (arrow) (e) and rods (f), labeled by WGA, revealed in red by CY3. Cells nuclei labeled by DAPI (blue). pl, photoreceptor layer; onl, outer nuclear layer; inl, inner nuclear layer; gcl, ganglion cell layer. Scale bar = 20 μm