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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Wnt signaling and polarity in freshwater sponges

Fig. 3

Expression of other Wnt pathway genes: fz1, fz2/4, fz3, dsh, gsk3, tcf. a-d), f) fz1, fz2/4, fz3, dsh and tcf mRNA expression, respectively (green), from fluorescent in situ hybridization with nuclear counterstain (magenta, blue or red, Hoechst) near choanocyte chambers. e) gsk3 expression in the same region, visualized with NBT/BCIP and sectioned in epoxy. g) Summary diagram of the sponge body plan (top view, left; side view, right) at 5 dph with cell types and regions shown where Wnt pathway gene expression was detected. The small inset on the periphery shows layers of different cell types and linings of sponge regions: the exopinacoderm (ex, blue), the endopinacoderm (en, red) and basopinacoderm (bp, green), between these layers is the mesohyl (mh, gray) which contains many cell types including archaeocytes. In the choanosome (inset, right), we find expression of fz, dsh, gsk3 and tcf. In the periphery (inset, left) we find wnts and β-cat. Modified from [61]. Scales: 100 μm

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