Fig. 3

Requirement of Dll and al in lateral branch formation in the B. mori antenna. (a-d’) Adult antennae subjected to RNAi against Dll (a, c-d’) and EGFP (b). (c) A SEM image of a Dll-RNAi antenna showing lack or substantial shortening of lateral branches and relatively normal segmentation and segment length. (c’) The magnified view of another Dll-RNAi antenna showing the region similar to the region boxed by a broken line in (c). Note small and fine protrusions (pseudo colored). (d, d’) A pupal Dll-RNAi antenna stained by the anti-Al antibody. The region boxed by a broken line in (d) is magnified in (d’). al expression is lost in large area (bracket) and remaining al expression is associated with small protrusion. (e-g’) al1-MO antennae of adult (e, f) and pupa (g, g’). The anti-Al antibody signal is shown by white (g) or magenta (g’) colors. (g’) is an image merged with the fluorescein signal (green) of MO. The SEM image (f) shows loss or reduced lateral branches (arrowheads). The anti-Al antibody staining shows loss of al expression (bracket). Penetrations of defective antennae are shown at lower-left corner in (a, b, e). Dashed lines and arrows in (d, g, g’) indicate the ventral midline and normal Al expression, respectively. Scale bars represent 2 mm in (a, b, e), 500 μm in (c), 200 μm in (c’, d, f-g’), 50 μm in (d’)