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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting

Fig. 2

The overlapping gene pair laoB/ECs5115 and the regions upstream and downstream. a Schematic view of the overlapping gene pair laoAB/ECs5115. Positions of the promoter, TSS, and transcription termination are indicated (not to scale). b Alignment of the proposed σ32 promoter to the consensus motif. c DNA sequence of the novel gene laoB and the upstream and downstream regions. The sequence of laoB is colored in blue and written in capital letters. The start codon is highlighted in green and the stop codon in red. Also the start and stop codon of the potential upstream gene laoA are marked (lower case, green and red, respectively). The TSS detected by 5’RACE is highlighted in pink and the transcriptional stop determined by 3’RACE is highlighted in yellow. The putative σ32 promoter is colored in orange. The SD sequence upstream laoB is highlighted in light blue

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