A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting

Table 1 Transcription and translation of laoB (part 1A) and its mother gene ECs5115 (part 1B) at the three different growth conditions indicated. The RPKM values of the transcriptome (RNAseq) and the translatome (RIBOseq) data for the overlapping novel gene and annotated mother gene are listed, including the RCV, indicating their translatability. ORF coverage is the fraction of a gene sequence, which is covered by RIBOseq reads. In addition, the corresponding data for the putative overlapping gene laoA (compare Fig. 2a) are shown (part 1C)

Condition	RPKM transcriptome^a	RPKM translatome^a	RCV^a	ORF coverage^a
1A laoB
LB, 37 °C	29.5	194	6.83	0.7
BHI, 37 °C	49.4	23.6	0.48	0.6
BHI + 4% NaCl, 14 °C	28.3	0.2	0.01	0.07
1B ECs5115
LB, 37 °C	19	12.3	0.65	0.35
BHI, 37 °C	26.9	7.1	0.27	0.45
BHI + 4% NaCl, 14 °C	10.2	0.6	0.07	0.16
1C laoA
LB, 37 °C	38.3	9.6	0.27	0.51
BHI, 37 °C	36.3	1.9	0.05	0.37
BHI + 4% NaCl, 14 °C	12.7	0.4	0.02	0.16

ISSN: 2730-7182