Fig. 2

Amino acid sequence alignment of PRDX5 orthologs in amniotes and Xenopus laevis amphibian. Multiple sequence alignment was performed with Clustal Omega, and displayed using GeneDoc with the conserved residue shading mode and similarity groups enabled. Letters on a black background represent residues conserved in 100% of the sequences at the corresponding position; residues shown on a gray background are conserved in > 80% of the sequences; residues shown on a light gray background are conserved in > 60% of the sequences. Mitochondrial targeting sequences (MTS) predicted by Mitoprot indicated in purple, and methionine residues aligning with human PRDX5 start methionines are in red. Conserved catalytic cysteines (peroxidatic cysteine, Cp; resolution cysteine, Cr) and peroxisomal targeting sequence (PTS) are indicated in yellow and green, respectively. Human PRDX5 protein secondary structure illustrated below the alignment is from Declercq et al. (2001) [71]. Accession numbers are as follow: NP_036226.1 (human, Homo sapiens); XP_006911206.1 (black flying fox, Pteropus alecto); XP_003230134.1 (anole lizard, Anolis carolinensis); XP_007434436.1 (Burmese python, Python bivittatus); KYO29582.1 (American alligator, Alligator mississippiensis); XP_005305567.1 (painted turtle, Chrysemys picta bellii); XP_018115535.1 (african clawed toad, Xenopus laevis). MTS of Python bivittatus was investigated using mRNA sequence XM_007434374.1 and WGS sequence NW_006534524.1. Alligator mississippiensis PRDX5 sequence was completed using sequences from the Alligator mississippiensis AKHW01000000 genome GBrowse