Fig. 5

CEACAM54 contains a P-T-R rich membrane proximal extracellular region. a Exon structure of the CEACAM54 gene and the two transcript variants identified in the present study. The simple sequence inserted in front of the transmembrane domain and the translation into the amino acid sequence (blue box) are depicted in (b). c Expression of transcript variant 1 (916 bp) in the intestine and trachea and of variant 2 (650 bp) in the vulva mucosa. Cartoon of the Flag-tag-CEACAM54 fusion protein (d). Expression of the Flag-tagged CEACAM54 at the cell surface of Cos7L cells as detected by flow cytometry (e). Red stars indicate the presence of a stop codon, mutated splice sites are marked with a red tilde