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Fig. 8 | BMC Evolutionary Biology

Fig. 8

From: Comparison of ventral organ development across Pycnogonida (Arthropoda, Chelicerata) provides evidence for a plesiomorphic mode of late neurogenesis in sea spiders and myriapods

Fig. 8

Ventral organs in the VNC of postlarval and juvenile P. litorale instars. Acetylated tubulin labeling shown in white – except for (a), which depicts cuticular autofluorescence in white. Nuclear counterstaining shown in blue. Maximum projections (a, c) and optical sections (b, df). Stippled ovals highlight the internal VOs of one body half. Stars indicate the central cavity around which tubulin-rich cell processes converge. White arrows indicate broad tubulin-rich cell bands and fibrous strands extending dorsally from the VOs towards the neuropil. White arrowheads mark the ventral longitudinal tracts. a Ventral overview of complete penultimate postlarval instar. b Wlg2–3, horizontal section. Black arrowheads highlight several mitoses in the VOs of wlg3. c First juvenile instar, sagittal vibratome section. d Wlg2, cross section. e Subesophageal ganglion, sagittal section. Black arrowhead marks potential pycnotic body indicative of cell death occurring in addition to cell proliferation. f Wlg2, sagittal section. Abbreviations: br – brain, cut – cuticle, ep – epidermis, mg – midgut, ot – ocular tubercle, ovn – ovigeral neuromere, pan – palpal neuromere, seg – subesophageal ganglion, wlg – walking leg ganglion, wln – walking leg neuromere

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