Fig. 3

Intron retention in G. sulphuraria. a Predicted changes resulting from the retention of 1152 alternatively spliced introns identified in this study. b Examples of intron retention in two G. sulphuraria genes. The sequences flanking the splicing signals (GT-AG) are illustrated. Coding nucleotides are shown in uppercase and non-coding nucleotides in lower case. Gray rectangular boxes are aligned with every other codon triplet. Gene models comprise thin black boxes (exons) connected with angled lines (introns). c Two examples of differential intron splicing between the heat and cold conditions in G. sulphuraria. Gene structures are shown at the top with the black boxes indicating the exons. For each gene, the read coverage along the displayed regions is shown for a total of 8 samples, with 2 replicates for each at the 4 temperature treatments (see Methods for details). The maximum read coverage is shown for each sample. For each intron, regions that are significantly more spliced than the compared temperature group are indicated with arrows