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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing

Fig. 3

Circos plots of horizontally transferred genes in HPA-evolved clones. a Horizontally transferred genes among \( \operatorname{Re}{\mathrm{c}}_{\mathrm{K}}^{\mathrm{B}} \) populations during HPA evolution. The circos plots show several concentric circles. The outermost circle (dark grey line) indicates genomic coordinates (in Mb) from the origin of replication (marked as 0), the location of the oriT located in the F-plasmid integrate, and the other two oriT sequences (blue rectangles). The innermost circle shows a radial black bar at each genomic location where a gene is present in the donor but not the recipient (K12) genome, as well as the location of the hpa operon in the E. coli REL606 B str. reference genome. The median circle (green bars) shows the number of populations that have acquired one or more genes from the B donor in at least one of the two clones sequenced (the maximum height of each green radial bar corresponds to six replicate populations). b Analogous to (a), except that the middle circle now reports the number of populations that have acquired genes (in E.coli B genome coordinates) that occur in both ancestral K recipient and the ancestral B donor. c and d, similar to (a) and (b), but for \( \operatorname{Re}{\mathrm{c}}_{\mathrm{K}}^{\mathrm{W}} \) populations. The innermost circle shows a radial black bar at each genomic location where a gene is present in the E. coli W but not the K12 genome. The orange bars show the number of populations that have acquired genes from the W donor in at least one of the two sequenced clones, for genes (c) that occur only in the W donor, and (d) that occur in both donor and recipient. Paa, the operon responsible for degrading aromatic compounds [56], is present in the W strain (see label at 4 o’clock) and the K strain, but not the B strain. All data are based on sequence coverage based estimation of horizontal gene transfer events (Methods)

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