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Fig. 4 | BMC Evolutionary Biology

Fig. 4

From: Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing

Fig. 4

Adaptation to butyric acid. a Fraction of butyric acid-adapted cells (vertical axis) for each of our four (six-fold replicated) experimental treatments (horizontal axis), as determined by a plating assay (Methods). Solid circles indicate data from each individual population (color legend). Box whisker plots display the median (central bar), the first and the third quartile (top and bottom bar of the box), and the range (whiskers) of a 95% interval of the fraction of cells able to form colonies on HPA. b Mean fitness of evolved populations (open diamonds, bars extend to one standard deviation from three biological replicates), and each of four clones isolated from each replicate population (solid circles, mean fitness from three biological replicates), measured as the growth rate in liquid medium supplemented with butyric acid. Box whisker plots display the median of mean fitness of clones (center bar), the first and third quartiles (box boundaries), and the range of a 95% interval of the data (whiskers). '\( \operatorname{Re}{\mathrm{c}}_{\mathrm{Y}}^{\mathrm{X}} \)' denotes populations of Y recipients exposed to donor X. Each replicate population within a treatment is labeled with a number and a distinct color in the legend. We note that the ancestors could not grow in butyric acid (Additional file 28: Figure S3), and fitness can thus not be given relative to the ancestor. Data is not shown for populations that had gone extinct during the experiment

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