Fig. 5

Circos plots of the distributions of horizontally transferred genes among butyric acid-evolved clones. a Putatively horizontally transferred genes among W recipients exposed to K donors in \( \operatorname{Re}{\mathrm{c}}_{\mathrm{W}}^{\mathrm{K}} \) experiments during adaptive evolution on butyric acid. The circos plots show several concentric circles. The outermost circle (dark grey line) indicates genomic coordinates (in Mb) from the origin of replication (marked as 0), the location of the oriT located in the F-plasmid integrate, and the other two oriT sequences (green rectangles). The innermost circle shows a radial black bar at each genomic location where a gene is present in the ancestral donor (K) but not the ancestral recipient (W) genome (in K genome coordinates). The middle circle shows the number of populations that have acquired one or more genes in at least one of the sequenced clones of the population at that location in the K donor, as the height of each green radial bar (maximum height corresponding to five populations). b Analogous to (a), except that the middle circle now reports the number of sequenced clones that have acquired the gene at that location only for genes that occur both in the ancestral W recipient and the ancestral K donor. Note that all gene locations are in coordinates of the E. coli K12 reference genome. All data are based on sequence coverage based estimation of horizontally transferred genes (Methods). The location of the ato operon (at 2.32 Mb), which is involved in butyric acid degradation is marked at the innermost circle