Table 2 Codon-based Z-test of selection for analysis averaging over all sequence pairs. Alternative hypotheses of neutrality, positive selection and purifying selection were tested for each population as well as for the total dataset of Carcinus aestuarii. For each population, examined specimens were assigned to the corresponding Cox1 type
Population | Number of specimens | Cox1 Type | Lack of neutral evolution (dN=/=dS) | Positive selection (dN > dS) | Purifying selection (dN < dS) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
I | II | III | Test statistic (dN-dS) | P value | Test statistic (dN-dS) | P value | Test statistic (dS-dN) | P value | ||
Tabarka | 10 | 10 | – | – | −2.381 | 0.019 | −2.509 | 1.000 | 2.403 | 0.009 |
Bizerte | 15 | 15 | – | – | −2.409 | 0.018 | −2.371 | 1.000 | 2.386 | 0.009 |
Sidi Rais | 11 | 10 | 1 | – | −4.545 | 0.000 | −4.538 | 1.000 | 4.631 | 0.000 |
Kelibia | 10 | 10 | – | – | −2.417 | 0.017 | −2.549 | 1.000 | 2.478 | 0.007 |
Benikhiar | 12 | 12 | – | – | −0.979 | 0.330 | −1.007 | 1.000 | 1.010 | 0.157 |
Monastir | 11 | 9 | 2 | – | −5.284 | 0.000 | −5.271 | 1.000 | 5.274 | 0.000 |
Chebba | 12 | 9 | 3 | – | −5.580 | 0.000 | −5.671 | 1.000 | 5.645 | 0.000 |
Sfax | 15 | 5 | 10 | – | −5.035 | 0.000 | −5.045 | 1.000 | 5.090 | 0.000 |
Djerba | 14 | 8 | 6 | – | −5.121 | 0.000 | −5.145 | 1.000 | 5.172 | 0.000 |
Tripoli | 11 | 10 | 1 | – | −4.672 | 0.000 | −4.755 | 1.000 | 4.818 | 0.000 |
Mosrata | 12 | 9 | 3 | – | − 5.216 | 0.000 | − 5.169 | 1.000 | 5.246 | 0.000 |
Venice Lagoon | 11 | 11 | – | – | −3.126 | 0.002 | −3.105 | 1.000 | 3.317 | 0.001 |
Pomer | 20 | 20 | – | – | −4.108 | 0.000 | −4.064 | 1.000 | 4.213 | 0.000 |
Amvrakikos | 13 | 13 | – | – | −3.480 | 0.001 | −3.554 | 1.000 | 3.514 | 0.000 |
Lefkada | 14 | 13 | – | 1 | −5.091 | 0.000 | −5.108 | 1.000 | 4.886 | 0.000 |
Navarino | 10 | 6 | – | 4 | −5.234 | 0.000 | −5.097 | 1.000 | 4.919 | 0.000 |
Alexandroupolis | 12 | – | – | 12 | −4.282 | 0.000 | −4.209 | 1.000 | 4.190 | 0.000 |
Izmir Bay | 6 | – | – | 6 | −2.023 | 0.045 | −2.089 | 1.000 | 2.084 | 0.020 |
Enez Dalyan Lagoon | 20 | – | – | 20 | −4.541 | 0.000 | − 4.577 | 1.000 | 4.540 | 0.000 |
Dardanelles Strait | 5 | – | – | 5 | −3.547 | 0.001 | −3.566 | 1.000 | 3.665 | 0.000 |
Prince’s Islands | 12 | – | – | 12 | −3.766 | 0.000 | −3.659 | 1.000 | 3.739 | 0.000 |
Bosphorus Strait | 7 | – | – | 7 | −2.649 | 0.009 | −2.599 | 1.000 | 2.626 | 0.005 |
Total | 263 | 170 | 26 | 67 | −6.224 | 0.000 | −6.013 | 1.000 | 6.256 | 0.000 |