Fig. 4

Relative fitness of mutations in glnG under nitrogen limiting conditions: Relative fitness of both glnG alleles (glnG V18L and glnG V18L G-7 T) under nitrogen limiting conditions was measured by competing each mutant against the wild-type. Relative fitness measurements of glnG G-7 T is also measured and is shown to be neutral under nitrogen limitation. Each experiment was done in a duplicate. Dotted line shows a relative fitness of 1, indicating neutral effect on fitness