Fig. 3

Cladograms of 117 angiosperms as shown in Fig. 1. Red open circles indicate loss of chloroplast RNA editing site rpoAeU200SF (in Ziziphus, Eucalyptus, Amaranthus tricolor, Bienertia and Silene latifolia). Blue open circles indicate loss of editing site clpPeU559HY (in Vicia, Cicer, Carica, Silene conica, Spirodela and in the Asterid clade). Both RNA editing sites simultaneously (green symbols) are absent in the Poales, in Amaranthus hypochondriacus, Camptotheca acuminata, Vaccinium macrocarpon in the Apocynaceae (Calotropis, Catharantus, Rauvolfia) and in Tarenaya hassleriana. No orthologues of editing factor CLB19 could be identified in Amaranthus hypochondriacus, Vaccinium macrocarpon and in Tarenaya hassleriana and, unexpectedly, also not in Amborella trichopoda and Spirodela polyrhiza (stippled lines). A more detailed analysis of Ericales (boxed) is given in Fig. 4. Also indicated is a parsimonious explanation for the presence of editing target ndhAeU473SL, which we discuss as an additional candidate target explaining retention of CLB19 in the Poales