Fig. 4

Nuclear localisation of Riox1 and Riox2. The N-terminal extension domain of human RIOX1 has been shown to harbour the nuclear localisation signal (NLS) [28]. a Expression of GFP-tagged full-length RIOX1 in HeLa cells resulted mainly in nucleolar accumulation. b A RIOX1 deletion mutant lacking aa 1–31 localised to the cytoplasm. c Fusion of aa 1–45 of human RIOX1 resulted in nuclear GFP localisation with strong accumulation in the nucleoli. DNA stain: DAPI. Scalebar: 5 μm. d Predictions of NLS in Riox1 of other species with either NLSmapper (blue) or NLStradamus (pink) identified NLS in the N-terminal extension domains of Riox1, whereas for Riox2 no NLS were predicted