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Fig. 8 | BMC Evolutionary Biology

Fig. 8

From: Reiterative use of FGF signaling in mesoderm development during embryogenesis and metamorphosis in the hemichordate Ptychodera flava

Fig. 8

The effect of FGF signaling on sand-induced metamorphosis. a-c The transforming rate of the Spengel larvae after 2 days of incubation with the sand and PD173074, U0126 (a), SU5402 (b) or bFGF protein (c) at indicated concentrations. The transformation rate was calculated by dividing the total number of the Spengel larvae used in the experiment with the sum up number of the Spengel larvae transformed into the Agassiz, transforming Agassiz and juvenile stages. d The percentages of the Spengel larvae that transformed into the Agassiz, transforming Agassiz, or juvenile stages after 2 days of incubation with sand, sterilized sand, or without sand in the presence of bFGF (+) or BSA (−) protein are shown. Every experiment was repeated at least three times except the U0126 treatment, which was conducted only once. N.S: not statistically significant

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