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Table 1 Functional characterisation of isolated Fads enzymes

From: Retention of fatty acyl desaturase 1 (fads1) in Elopomorpha and Cyclostomata provides novel insights into the evolution of long-chain polyunsaturated fatty acid biosynthesis in vertebrates

FA

Substrate

FA

Product

% Conversion

Lca

Fads1

Lca

Fads2

Loc

Fads1

Loc

Fads2

Pse

Fads1

Pse

Fads2

Aja

Fads1

Ajaa

Fads2

Pbu

Fads2A

Pbu

Fads2B

Activity

18:3n-3

18:4n-3

n.d.

6.6

n.d.

32.4

n.d.

37.1

n.d.

64.3

77.4

n.d

Δ6

18:2n-6

18:3n-6

n.d.

2.0

n.d.

15.6

n.d.

20.6

n.d.

20.7

42.7

n.d

Δ6

20:3n-3

20:4n-3

n.d.

0.7

n.d.

4.1

n.d.

11.0

n.d.

6.0

18.4

n.d

Δ8

20:2n-6

20:3n-6

n.d.

n.d.

n.d.

1.5

n.d.

3.6

n.d.

5.4

7.0

n.d.

Δ8

20:4n-3

20:5n-3

6.0

n.d.

3.0

n.d.

56.1

n.d.

58.1

n.d.

n.d.

14.4

Δ5

20:3n-6

20:4n-6

5.5

n.d.

2.9

n.d.

48.3

n.d.

33.2

n.d.

n.d.

11.7

Δ5

22:5n-3

22:6n-3

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Δ4

22:4n-6

22:5n-6

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Δ4

Overall activity

Δ5

Δ6/Δ8

Δ5

Δ6/Δ8

Δ5

Δ6/Δ8

Δ5

Δ6/Δ8

Δ6/Δ8

Δ5

 
  1. The conversions were calculated according to the formula [product area/(product area + substrate area) × 100]
  2. Lca Lethenteron camtschaticum, Loc Lepisosteus oculatus, Pse Polypterus senegalus, Aja Anguilla japonica, Pbu Pantodon buchholzi, n.d. indicates not detected
  3. aData collected from [6]