Fig. 6

CRISPR/cas9 knockout of nucleoplasmin (npm) 2a and npm2b in zebrafish. (a) Normalized expression level of npm2a and npm2b transcripts by quantitative real-time PCR (qPCR) in the fertilized zebrafish eggs from crosses between npm2a mutant, npm2b mutant, or wildtype (WT) (control) female and WT male, respectively. (b) Developmental success as measured by the proportion of fertilized eggs that underwent normal cell division and reached normal developmental milestones based on Kimmel et al. [23] from crosses between WT animals (control), and npm2a or npm2b mutant female and WT male at 1 and 24 h post-fertilization (hpf). qPCR data were normalized to 18S, β-actin, β2-microglobulin, and ef1α. N = 4 each for npm2a mutant and control, and N = 5 for npm2b mutant. All assessments were performed from at least 3 clutches from each mutant. **p < 0.01, ***p < 0.001, ****p < 0.0001 by Mann-Whitney U-test