Skip to main content

Table 3 Expression analysisa of selected Clonostachys rosea S8A serine protease genes during interaction with fungal prey

From: Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea

 

prs1

prs2

prs3

prs4

prs5

prs6

prs7

prs8

prs9

prs10

prs11

prs12

rs13

prs14

prs15

prs16

prs17

prs18

Cr-Fg

0.57 a

0.43 b

0.49 b

0.52 a

N/D

1.47 a

1.74 a

N/D

0.63 ab

N/D

0.53 b

N/D

0.48 b

0.53 b

1.54 a

0.84 a

N/D

1.48 a

Cr-BC

1.01 a

0.35 b

0.74 ab

0.77 a

N/D

1.26 ab

1.04 a

D

0.33 b

N/D

0.56 b

N/D

0.94 ab

0.24 b

0.83 b

0.71 a

1.29 a

0.98 a

Cr-Cr

1.27 a

1.08 a

1.03 a

1.13 a

N/D

1.01 b

1.24 a

N/D

1.15 a

D

1.02 a

N/D

1.10 a

1.23 a

1.12 ab

1.04 a

8.26 a

1.11 a

  1. N/D no detectable expression, D detectable expression
  2. aGene expression of S8A serine protease genes was determined by RT-qPCR during the interaction of C. rosea with B. cinerea (Cr-Bc), C. rosea with F. graminearum (Cr-Fg) and C. rosea with itself (Cr-Cr, control). Relative expression is calculated as the ratio between the target gene and tubulin using the 2–ΔΔCT method. Different letters indicate significant differences (P ≤ 0.05) between treatments for each gene as determined by the Fisher’s least significant difference (LSD) test. The statistical analysis was performed on a minimum of three biological replicates