Skip to main content
Fig. 5 | BMC Evolutionary Biology

Fig. 5

From: Variations in the breeding behavior of cichlids and the evolution of the multi-functional seminal plasma protein, seminal plasma glycoprotein 120

Fig. 5

Glycosylation of SPP120 is major protein in seminal plasma and involved in milt viscosity in the oral fertilization species Oreochromis mossambicus (a) Maps of seminal plasma proteins (Coomassie brilliant blue staining), N-glycosylated proteins (ConA lecting blotting), and Anti-SPP120 antibody reacted protein in the oral fertilization species Oreochromis mossambicus. Glycopeptidase-F treatment was applied to seminal plasma, and its effect on b milt viscosity and c the glycosylation states of SPP120 and was observed. Milt diffusion rates were calculated as the ratio of the diffused area just after glycopeptidase-F inoculation and 3 s later (N = 3) and compared between control and peptidase-treated milt (t-test d.f. = 2.05, t = 6.52, P < 0.02)

Back to article page

BMC Ecology and Evolution

ISSN: 2730-7182

Contact us