Fig. 6

Differences in milt diffusion rates and glycosylation states of SPP120 among the fertilization types of cichlids from Lake Tanganyika. a Milt diffusion was evaluated in seven species (see also Additional file 11: Figure S4) and compared (Phylogenetic generalized least squares P < 0.005; see also Additional file 10: Table S3). Oral fertilization species: Or. mossambicus (N = 3), Petrochromis fasciolatus (N = 4), and Ophthalmotilapia ventralis (N = 4); Stocked-spermatozoa fertilization species: Cyathopharynx furcifer (N = 3) and Op. nasuta (N = 3); and substrate fertilization species: Variabilichromis moorii (N = 3), and Lepidiolamprologus elongatus (N = 3). b Glycosylation states of SPP120 in these seven species are also shown. c Transitions among glycosylation states were calculated in BayesTraits version 2.0 according to maximum likelihood estimations. Arrows indicate transition rates (O₀₁, Q₁₀), which are an indicator of how likely a particular transition is to have occurred. Q₀₁ indicates rates from non-glycosylated SPP120 to glycosylated one, Q₁₀ is from glycosylated SPP120 to non-glycosylated one. Histograms of each transition rate are also shown