Fig. 2

Analysis of early-diverging lineages provides evidence of ancestral septin duplications. a) Shaded area of pie charts indicates the proportional likelihood that a specific ancestor had a member of a septin group, when reconstructed under maximum likelihood. As Group 2 may or may not be monophyletic, reconstructions resulting from these two alternatives are illustrated. b) Gene copy number reconstructed within the species phylogeny. Branch thickness represents the average NOTUNG inferred number of septin gene copies based on the jPRIME septin gene phylogeny. Stars indicate a change in morphology of organisms in a lineage. Note: This species phylogeny was used to guide the jPRIME analysis. c) Cell shading indicates copy numbers of genes representing each septin group, classified by each of three phylogenetic methods. Classification of septins from an organism sometimes differed depending on the analysis method, reflecting uncertainty in phylogenetic placement of divergent sequences