Fig. 7

Expression profile of ogn1 and ogn2 in gilthead sea bream bone-derived primary MSCs cultures in adipogenic conditions. (a) Representative images of gilthead sea bream cells from vertebra growing in adipogenic medium (AM) at days 10 and 20 of culture development. The images were acquired using a 20x magnification and a scale bar is also indicated in the image (100 μm). Normalized expression (copy number) of (b) ogn1 and (c) ogn2 in bone-derived cells growing in control (GM) or AM at different days of the culture (5 to 20) determined using the geometric mean of the reference genes ef1α and rps18. Results are shown as the mean ± sem (N = 5–6 independent cultures). A two-way ANOVA followed by the Fisher’s Least Significant Difference (LSD) post-test was performed to identify differences in gene expression among the experimental groups. Significant differences (p < 0.05) between culture conditions are represented with an asterisk