Fig. 8

Expression profile of ogn1 and ogn2 in gilthead sea bream myocyte primary cultured cells. (a) Representative images of gilthead sea bream muscle cells at days 2 and 8 of culture development. The images were acquired using a 20x magnification and a scale bar is also indicated in the image (100 μm). Normalized expression (copy number) of (b) ogn1 and (c) ogn2 in the myocyte cells at different culture days (2 to 12) determined using the geometric mean of the reference genes ef1α and rps18. Results are shown as the mean ± sem (N = 4 independent cultures). A One-way ANOVA followed by a Tukey test was used to test for significant differences in gene expression through time and are indicated with letters (different letters denote significant differences, p < 0.05)