Fig. 4

Mutations identified in the 4 evolved strains compared to the original strain Genr0. a The genes and intergenic regions with SNP mutations are shown. For coding regions (in black) the resulting amino acid substitution is indicated. The dotted inner ring indicates the genome position. The rings are color-coded according to the COG-classification of the gene present on the forward and reverse strands, and from inner to outer ring we represent evolved strains 445C1 to C4. b The DNA mutations in the codon for Met19 in CcpA are shown for each strain