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Fig. 8 | BMC Evolutionary Biology

Fig. 8

From: Adaption to glucose limitation is modulated by the pleotropic regulator CcpA, independent of selection pressure strength

Fig. 8

Abundance of the three CcpA species found during evolution experiment. ccpA accumulates the Met19 to Ile early in evolution experiment and quickly takes over the population. Indicated are the averages of two pyrosequencing reactions and in the case of strain 445C4, the abundance of the Thr mutation was verified by Sanger sequencing. The PCR was performed from the frozen stocks sampled during the continuous culture process. In green is indicated the prevalence of the Ile mutant, in blue wild type CcpA and in grey the Thr mutant. a 445C1, b 445C2, c 445C3, d 445C4

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