Table 2 Primers used for gene amplification and sequencing
From: Nature of selection varies on different domains of IFI16-like PYHIN genes in ruminants
Gene | Primer name | Sequence (5′ – 3′) | Tm (°C) | Length (bp) |
|---|---|---|---|---|
IFI16-L | IFI16FW (Full ORF) | AGCCAGCACTAAATCAAGGGTTTTGACTAGTC | 62 | 32 |
IFI16RV (Full ORF) | GCCAAGTTATAAAAGTTATAGATGCAAAATGGGACA | 60 | 36 | |
IFI16FW2 | GTGACACAGCCACCAAACCTAAGGAC | 61 | 26 | |
IFI16FW3 | GAGGCACATCCCTAATTTGAGAGATGGGG | 63 | 29 | |
IFI16RV1 | ATCTGGCCACTGCTCACAGTTGGG | 61 | 24 | |
M13 | M13/pUC | GTAAAACGACGGCCAGT | 47 | 17 |
M13/pUCRV | CAGGAAACAGCTATGAC | 45 | 17 |