Fig. 8

Expression of Sox D, E and F group orthologues. Flat-mounted embryos at different stages of development after RNA in situ hybridization. a) ptSoxD-1 expression is observed throughout the ventral nerve cord in stage 10 embryos as indicated by the arrows. b) ptSoxE-1 expression at stage 9 is visible as single foci in the forming chelicerae, broader expression in the pedipalps and L1 to L3 (white arrows), and as two strong foci in the L4 limb buds (black arrowhead). c) The expression of ptSoxF-2 is only visible in the L1 limb buds forming at stage 9 (arrows). Ch: chelicerae, L1 – L4: prosomal segments 1 to 4, O1 – O4: opisthosomal segments 1 to 4, Pp: pedipalps; SAZ: segment addition zone. Ventral views are shown for all embryos with the anterior to the left. Scale bars: 150 μm