Table 1 nSSR summary information on each locus
Locus | Nucleotide repeat | Size (bp) | Primer sequence | nA | Ta (°C) | Pc (μM) | HO | HS | p |
|---|---|---|---|---|---|---|---|---|---|
Rrms18 | CATT | 130–143 | F: GCTTTCATTGTTGTACACCTC | 4 | 53 | 3 | 0.171 | 0.488 | <0.001 |
R: ACAAACAGCAATGAGGTACAG | |||||||||
Rrms34 | TGAA | 106–136 | F: AATAATGTTTCGCACTGAGAG | 11 | 53 | 15 | 0.748 | 0.772 | 0.183 |
R: CAAGGTAGACCGTTACAGTGA | |||||||||
Rrms40 | CACT | 85–118 | F: GTAATGGCCATGTCACTAGC | 9 | 53 | 10 | 0.246 | 0.577 | <0.001 |
R: TTTGAGACTCGAAAGAAACAG | |||||||||
Rrms44 | GAGT | 91–98 | F: CTATGTTGAAAAGGCATCAAT | 3 | 51 | 15 | 0.438 | 0.404 | 0.108 |
R: GCAAAGTGTTGTTCACTCAAT | |||||||||
Rrms72 | CATT | 128–142 | F: GAAATGTCAAAGACGAAAGTG | 8 | 51 | 15 | 0.707 | 0.711 | <0.05 |
R: TTGAAGTGCGAAATTAGTCAT | |||||||||
Rrms91 | GAGT | 84–92 | F: CTATGTTGAAAAGGCATCAAT | 4 | 51 | 5 | 0.587 | 0.625 | <0.001 |
R: GCAAAGTGTTGTTCACTCAAT | |||||||||
Rrms03 | AATA | 147–149 | F: AACTTGGTCTAAAGTGAAGCA | 2 | 53 | 5 | – | – | – |
R: TTGAAAAGTCACTAAGCCAAC | |||||||||
Rrms23 | CTCC | 141–142 | F: CCGTAATGTACGACAAAGTGT | 2 | 53 | 15 | – | – | – |
R: AAGGTAATCTATCCCCCACT | |||||||||
Rrms61 | CGA | 74–76 | F: TAAATAGATCGAGACGACCAA | 2 | 53 | 15 | – | – | – |
R: TCTCTGTGTGAACGATCTGTA |