Fig. 4

Asymmetry of ancestral state retention in TF WGD-duplicates. a Example of how Asymmetry score (Asy, see Methods) is calculated. Ancestral conditions are indicated by yellow boxes and non-ancestral conditions by grey boxes. Among a pair of duplicates, an ‘ancestral’ copy (red arrow) is the duplicate retains more ancestral states than the other, ‘non-ancestral’ copy (blue arrow). In case where equal numbers of ancestral states are inherited (the first case with Asy = 0), the ancestral and non-ancestral designation is assigned randomly. b The Asymmetry scores of ancestral expression partitioning between TF WGD-duplicates. Red columns indicate the expected frequency of each score bin based on a series of grouped Bernoulli trials (see Methods) while blue columns indicated the observed frequency. c The Asymmetry scores of ancestral cis-regulatory site partitioning between TF WGD-duplicates. Red and blue columns are as described in (b). d The frequency distribution of the difference in number of novel cis-regulatory sites between ancestral and non-ancestral WGD duplicate copies. The value on the x-axis is calculated as the number of novel regulatory sites in the non-ancestral copy minus the number in the ancestral copy