Fig. 7
Analysis of the Ms1 and 6C RNA genes. a Gene synteny plot for the region encoding Ms1 and 6C RNA genes in selected Actinomycetes as indicated. The Ms1 RNA gene is marked in red (orange in Streptomyces coelicolor), while the 6C RNA gene is marked in green. The vertical boxes marked in brown highlight homologous genes. b Gels showing levels of 5S rRNA, Ms1 RNA and 6C RNA from three independent experiments in stationary and exponentially growing MmucT and MaubT cells as indicated. Note the presence of the extra band, marked as band 1, in one of the experiments. The bottom panel represents overexposure of the Ms1 RNA bands from exponentially growing MmucT cells. c Levels of Ms1 RNA and 6C RNA in stationary cells vs exponentially growing cells in MmucT as indicated using data presented in (b). The experiments were performed as outlined in Methods and values represent the mean and errors based on three independent experiments. Statistical significance using Student’s unpaired, two-tailed t-test; **: p < 0.01; ***: p< 0.001. d Predicted secondary structure for MmucT Ms1 RNA. The boxes mark regions that vary in sequence comparing MmucT, MphoT, MaubT, MneoT and McosT (see Additional file 4: Figure S12b). Helices and loops are named P1 to P7 and L1 to L3 as indicated. e Predicted secondary structure for MmucT 6C RNA. The helices and loops are named as in (d), see also Additional file 4: Figure S12d