Fig. 2

Designation of eForbs (EP < 0.05) using the original and modified EP methods. Agreement for classification of evolutionary forbidden alleles (eForbs) using the original and modified EP calculated methods for (a) all missense variants found in 1000 Genomes Project Phase III dataset, (b) human disease associated missense variants found in the HGMD disease variation dataset, and (c) high allele frequency (global AF > 5%) missense variants with EP < 0.05 (CAPs). Single darkened circles under a bar represent eForbs identified by the indicated method, and not the other. Connected darkened circles represent eForbs identified by both methods