Fig. 5
The numbers of genetic changes acquired by the completely denitrifying clones (one clone per culture) compared to the sums of genetic changes acquired by the nitrite (NO2−) producing and reducing clones (one clone each per culture) after 700 generations of evolution at pH 6.5 (strong nitrite toxicity, strong interdependence). The horizontal bars and P-values indicate the outcomes from two-sample Wilcoxon rank-sum tests. The star indicates a P-value less than 0.05. Data are presented as Tukey box-plots. The numbers of genetic changes include synonymous and non-synonymous point mutations, insertions, deletions, and multiplications. Panels are for a data excluding the clone with a genetic change in uvrA, and b data including the clone with a genetic change in uvrA (indicated by the red arrow). The sums of genetic changes acquired by the nitrite producing and reducing clones are greater than the numbers of genetic changes acquired by the completely denitrifying clones, but only when the clone containing a genetic change in uvrA is removed from the analysis