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Table 1 Ascorbate peroxidase purification from P. marinus

From: Lacking catalase, a protistan parasite draws on its photosynthetic ancestry to complete an antioxidant repertoire with ascorbate peroxidase

Enrichment Step

Total protein

mg

Specific activity

μM min− 1 mg− 1

Yield

%

Purification

-fold

Osmotic lysis

16.1

0.49

100

1.0

DEAE sephacel

2.85

6.63

238a

13.7

Phenyl sepharose

0.033

493.5

206a

1007

  1. aYield of > 100% may reflect the removal of an inhibitor in the DEAE step