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Table 3 Primers used for PCR and sequencing in this study

From: A newly discovered radiation of endoparasitic gastropods and their coevolution with asteroid hosts in Antarctica

Primer Name (F/R)

Marker

F/R Sequence (5′ to 3′)

Reference

LCO1490/ HCO1490

COI parasites

GGTCAACAAATCATAAAGATATTGG/

TAAACTTCAGGGTGACCAAAAAATCA

[59]

COIceF/ COIceR or jgLCO1490/ jgHCO2198

COI hosts

ACTGCCCACGCCCTAGTAATGATATTTTTTATGGTN-ATGCC/ TCGTGTGTCTACGTCCATTCCTACTGTRAA-CATRTG TITCIACIAAYCAYAARGAYATTGG/ TAIACYTCIGGRTGICCRAARAAYCA

[60, 61]

16SarL/ 16SbrH

16S

CGCCTGTTTATCAAAAACAT/ CCGGTCTGAACTCAGATCACGT

[62]

H3MF/H3MR

H3

ATGGCTCGTACCAAGCAGACTGC/ TGGATGTCCTTGGGCATGATTGTTAC

[63]

ANTF/ANTR1/ ANTR2

ANT

CCATTYTGGMGIGGWAACWTGGC/ TTCATCAAIGACATRAAICCYTC/ CCCTTGTAYTTRACASCYTCACC

[64]

1100F (forward) na2 (reverse) with

28S

GGACCCGAAAGATGGTGAACTATGC/ AGCCAATCCTTATCCCGAAG

[25, 65]

FL (forward)*

 

AAGTGGAGAAGGGTTCCATGT

[25]

LSU5/ LSU1600R with ECD25 (reverse)*

 

TAGGTCGACCCGCTGAAYTTAAGCA/ AGCGCCATCCATTTTCAGG CTTGGTCCGTGTTTCAAGACGG

[66, 67]

[68]

  1. Multiple primer sets were used to amplify the entire fragment of 28S, with an asterisk denoting sequencing primers
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BMC Ecology and Evolution

ISSN: 2730-7182

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