Fig. 5

Difference in mutational neighborhood of the mutator clones between generation 390,000 and 300,000. For each mutator lineage 2 million offspring were generated from the ancestor at generation 300,000 and 2 million from the ancestor at generation 390,000 (see “Methods”). For each offspring, we measured Δg, the variation of metabolic error relatively to its parent. We then binned these differences in 6 bins and measured the variation of frequency in these bins between generations 300,000 and 390,000 for each lineage (Stars). Areas represent the maximum density of presence in the plot. We observe a slight increase in the number of offspring that gain fitness relatively to their parent (negative variation of metabolic error), no variation of neutral offspring, a decrease in slightly deleterious offspring (bin 0.00025) and an increase in highly deleterious offspring (bin 0.001)