Fig. 2

Positively selected residues of Ilex represented on the surface of the Rubisco large subunit. a Front and back of folded large subunit of the modeled Ilex canariensis 3-D structure. Visible positively selected residues from the Worldwide dataset are in red, while additional positively selected residues from the Chinese dataset are in blue. b Extrapolation on the Rubisco Spinacia oleracea 3-D structure (1RCX). Presented are the 4 large subunits (E,B,V and R) and 3 small subunits (f, s and w) that are in contact with one large subunit (here L, in orange). Positively selected residues are represented with the same colors as in A. Most of these residues (represented here on the L subunit) are exposed on the equatorial surface the L₈S₈ Rubisco hexadecamer. Sites 142, 429 and 461 are involved in intra-dimer, dimer-dimer and L/S interactions (see Table 3). All other sites (15, 19, 21, 23, 28, 30, 84, 86, 91, 95, 97, 340, 353, 354 and 470) represent positively selected residues potentially involved in interactions with chaperones