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Fig. 2 | BMC Evolutionary Biology

Fig. 2

From: Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits

Fig. 2

Western blotting for YFP-VMAT1 and its variants. Cells were transfected with plasmids encoding YFP, YFP-VMAT1, or its variants. Cells without transfection (no transfection) were also prepared (lane 1). Cell lysates were analyzed by immunoblotting with anti-GFP, anti-VMAT1, and anti-α-tubulin antibodies. Immunoblotting of α-tubulin is shown as a loading control. The major immunoreactive bands of GFP and VMAT1 at nearly 80 kDa are consistent with the putative molecular weight of the YFP-VMAT1 fusion protein. The full-length blots for all cropped ones are shown in (Additional file 1: Figure S1a)

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BMC Ecology and Evolution

ISSN: 2730-7182

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