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Table 4 Rubisco kinetic parameters at 25 °C and candidate positively selected sites in the Rubisco L-subunit

From: Exploring molecular evolution of Rubisco in C3 and CAM Orchidaceae and Bromeliaceae

SpeciesPhotosynthetic typeKckcatckcatc/Kc[Rubisco]/
[TSP]
Candidate L-subunit residuesunder positive selection
Orchids     89225251265449461468470477478479481
 Sobralia macranthaC312.1 ± 1.4a2.5 ± 0.8ab0.21 ± 0.08a8.9 ± 1.5aVLIVSINEQLD
 Elleanthus furfuraceusC312.4 ± 0.7a2.8 ± 0.3ab0.22 ± 0.01a7.2 ± 0.7a.I......K..
 Oncidium lineoligerumC314.4 ± 2.3a2.9 ± 0.2abc0.21 ± 0.02a7.2 ± 2.1aA.....DD.ET
 Myoxanthus exasperatusC321.3 ± 1.3b3.7 ± 0.2bcd0.18 ± 0.01a7.6 ± 2.1a...........E
 Lockhartia amoenaC323.4 ± 0.4b4.3 ± 0.1cd0.18 ± 0.01a6.3 ± 0.3a.I..A......
 Maxillaria cucullataC324.2 ± 4.1b4.4 ± 0.9d0.19 ± 0.01a6.0 ± 0.9a..M........
Bromeliads     2891142225251449468478    
 Tillandsia bifloraC39.6 ± 0.7a2.3 ± 0.2a0.25 ± 0.03a18.0 ± 1.1cDVPIICDT    
 Tillandsia multicaulisC311.8 ± 0.6a3.2 ± 0.1a0.27 ± 0.02a6.2 ± 0.4b......EA    
 Nidularium innocentii var. lineatumC315.5 ± 0.5a3.4 ± 0.6a0.22 ± 0.04a7.2 ± 0.9 bEL.L.S..    
 Tillandsia bermejoensisStrong CAM23.1 ± 3.4b5.7 ± 2.0a0.24 ± 0.06a1.4 ± 0.3a........    
 Aechmea nudicaulis var. aureo-roseaStrong CAM27.4 ± 2.2b6.4 ± 2.0a0.26 ± 0.09a1.1 ± 0.2a........    
 T. aestivum cv. CajemeC310.3 ± 0.3a1.9 ± 0.1a0.18 ± 0.01a20.9 ± 0.8d            
  1. Rubisco kinetics for 6 orchids, 5 bromeliads and Triticum aestivum. Rubisco Michaelis-Menten constant for CO2 (Kc, μM), maximum rate of carboxylation (kcatc, s−1), carboxylase catalytic efficiency (kcatc/Kc, s−1 μM−1), and Rubisco per leaf total soluble protein ([Rubisco]/[TSP], %). Data are mean ± S.E. (n = 3). Different letters denote statistically significant differences among species within orchids and bromeliads through Duncan test (p < 0.05). The photosynthetic mechanism is indicated for each species according to Table 1 and Additional file 1: Table S1. The sequence of T. aestivum was taken from GenBank (Accesion number KJ592713) for comparison. Residues identical to those of the first sequence are shown as dots