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Table 4 Rubisco kinetic parameters at 25 °C and candidate positively selected sites in the Rubisco L-subunit

From: Exploring molecular evolution of Rubisco in C3 and CAM Orchidaceae and Bromeliaceae

Species

Photosynthetic type

Kc

kcatc

kcatc/Kc

[Rubisco]/

[TSP]

Candidate L-subunit residuesunder positive selection

Orchids

     

89

225

251

265

449

461

468

470

477

478

479

481

 Sobralia macrantha

C3

12.1 ± 1.4a

2.5 ± 0.8ab

0.21 ± 0.08a

8.9 ± 1.5a

V

L

I

V

S

I

N

E

Q

L

D

–

 Elleanthus furfuraceus

C3

12.4 ± 0.7a

2.8 ± 0.3ab

0.22 ± 0.01a

7.2 ± 0.7a

.

I

.

.

.

.

.

.

K

.

.

–

 Oncidium lineoligerum

C3

14.4 ± 2.3a

2.9 ± 0.2abc

0.21 ± 0.02a

7.2 ± 2.1a

A

.

.

.

.

.

D

D

.

E

T

–

 Myoxanthus exasperatus

C3

21.3 ± 1.3b

3.7 ± 0.2bcd

0.18 ± 0.01a

7.6 ± 2.1a

.

.

.

.

.

.

.

.

.

.

.

E

 Lockhartia amoena

C3

23.4 ± 0.4b

4.3 ± 0.1cd

0.18 ± 0.01a

6.3 ± 0.3a

.

I

.

.

A

.

.

.

.

.

.

–

 Maxillaria cucullata

C3

24.2 ± 4.1b

4.4 ± 0.9d

0.19 ± 0.01a

6.0 ± 0.9a

.

.

M

.

.

.

.

.

.

.

.

–

Bromeliads

     

28

91

142

225

251

449

468

478

    

 Tillandsia biflora

C3

9.6 ± 0.7a

2.3 ± 0.2a

0.25 ± 0.03a

18.0 ± 1.1c

D

V

P

I

I

C

D

T

    

 Tillandsia multicaulis

C3

11.8 ± 0.6a

3.2 ± 0.1a

0.27 ± 0.02a

6.2 ± 0.4b

.

.

.

.

.

.

E

A

    

 Nidularium innocentii var. lineatum

C3

15.5 ± 0.5a

3.4 ± 0.6a

0.22 ± 0.04a

7.2 ± 0.9 b

E

L

.

L

.

S

.

.

    

 Tillandsia bermejoensis

Strong CAM

23.1 ± 3.4b

5.7 ± 2.0a

0.24 ± 0.06a

1.4 ± 0.3a

.

.

.

.

.

.

.

.

    

 Aechmea nudicaulis var. aureo-rosea

Strong CAM

27.4 ± 2.2b

6.4 ± 2.0a

0.26 ± 0.09a

1.1 ± 0.2a

.

.

.

.

.

.

.

.

    

 T. aestivum cv. Cajeme

C3

10.3 ± 0.3a

1.9 ± 0.1a

0.18 ± 0.01a

20.9 ± 0.8d

            
  1. Rubisco kinetics for 6 orchids, 5 bromeliads and Triticum aestivum. Rubisco Michaelis-Menten constant for CO2 (Kc, μM), maximum rate of carboxylation (kcatc, s−1), carboxylase catalytic efficiency (kcatc/Kc, s−1 μM−1), and Rubisco per leaf total soluble protein ([Rubisco]/[TSP], %). Data are mean ± S.E. (n = 3). Different letters denote statistically significant differences among species within orchids and bromeliads through Duncan test (p < 0.05). The photosynthetic mechanism is indicated for each species according to Table 1 and Additional file 1: Table S1. The sequence of T. aestivum was taken from GenBank (Accesion number KJ592713) for comparison. Residues identical to those of the first sequence are shown as dots