Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae)

Table 1 List of primer sequences and references used for molecular phylogenetic analyses and conditions for PCR

Target sequence	Forward and reverse primer names	5′ – 3′ primer sequence	Reference	Temperature (°C) and durations (sec) of denaturation, annealing and extension and total number of cycles	Magnesium concentration (mM)
trnL-F	c	CGA AAT CGG TAG ACG CTA CG	Taberlet et al. [86]	94, 60; 55, 60; 72, 120; 30	2
	f	ATT TGA ACT GGT GAC ACG AG	Taberlet et al. [86]
psbM-trnD^GUCspacer	trnD^GUCR	GGG ATT GTA GYT CAA TTG GT	Shaw et al. [87]: modified from Demesure et al. [88]	94, 60; 55, 60; 72, 210; 35	2
	psbMF	AGC AAT AAA TGC RAG AAT ATT TAC TTC CAT	Shaw et al. [87]
rps16	rpsF	GTG GTA GAA AGC AAC GTG CGA CTT	Oxelman et al. [89]	95, 30; 60, 60; 72, 120; 33	2
	rpsR2	TCG GGA TCG AAC ATC AAT TGC AAC	Oxelman et al. [89]
matK-5′trnK spacer	matK6	TGG GTT GCT AAC TCA ATG G	Johnson and Soltis [90]	94, 60; 50, 60; 72, 90; 35	2
	matK5′R	GCA TAA ATA TAY TCC YGA AAR ATA AGT GG	Shaw et al. [87]
trnC^GCA-ycf6 region	ycf6R	GCC CAA GCR AGA CTT ACT ATA TCC AT	Shaw et al. [87]	94, 60; 50, 60; 72, 210; 35	2
	trnC^GCAF	CCA GTT CRA ATC YGG GTG	Shaw et al. [87]
rps4-trnT spacer	trnT^UGUR	AGG TTA GAG CAT CGC ATT TG	Shaw et al. [87]	92, 60; 55, 60; 72, 180; 30	2.5
	rps4R2	CTG TNA GWC CRT AAT GAA AAC G	Shaw et al. [87]
ITS	ITS1	TCC GTA GGT GAA CCT GCG G	White et al. [91]	94, 90; 55, 70; 72, 90; 30	2
	ITS4	TCC TCC GCT TAT TGA TAT GC	White et al. [91]

ISSN: 2730-7182