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Table 1 Flat periwinkle samples analyzed in this study. Shown is the locality and country of origin, together with year of collection, geographical coordinates and habitat type. Locality codes include “o” or “f” as prefix when corresponding to L. obtusata or L. fabalis samples, respectively, followed by the initials of the country where samples were collected (Fig. 1). N: sample size (specified by sex: mal, males; fem, females), as the initial number of individuals considered for analysis, followed by the final number of individuals retained in each dataset (COI + Cyt-b: concatenated cytochrome oxidase subunit I and cytochrome b; Thio: thioredoxin peroxidase 2; Cal: calreticulin)

From: Phylogeographic history of flat periwinkles, Littorina fabalis and L. obtusata

Locality

Country

Year of

Coordinates

Wave Exposure /

Code

N Initial

N COI + Cyt-b

N Thio

N Cal

  

Collection

Latitude

Longitude

Habitat

 

(mal/fem)

(mal/fem)

(mal/fem)

(mal/fem)

L. obtusata

Nahant

Massachusetts (USA)

2005

42°25′41.22″N

70°55′43.26″W

NA

(1) oUS1

12 (NA)

10 (NA)

11 (NA)

8 (NA)

Quoddy Head

Maine (USA)

2005

44°48′49.74″N

66°57′3.66″W

NA

(2) oUS2

12 (NA)

8 (NA)

11 (NA)

6 (NA)

Sandgerdi

Iceland

2005

64° 2′0.27″N

22°44′11.82″W

NA

(3) oIC

12 (NA)

10 (NA)

11 (NA)

7 (NA)

Shetland Islands

Scotland (UK)

2007

59°53′6.04″N

1°17′10.71″W

NA

(4) oSC

12 (NA)

9 (NA)

12 (NA)

1 (NA)

Seløyna 1

Norway

2012

60°38′13.62″N

4°47′33.72″E

Sheltered

(5) oNO1

29 (12/17)

15 (9/6)

11 (9/2)

8 (7/1)

Seløyna 2

Norway

2012

60°38′7.08″N

4°47′32.94″E

Exposed

(6) oNO2

4 (2/2)

3 (1/2)

3 (1/2)

3 (1/2)

Lökholmen 1

Sweden

2012

58°53′21.98″N

11° 6′38.61″E

Sheltered

(7) oSW1

12 (9/3)

12 (9/3)

11 (8/3)

10 (7/3)

Lökholmen 2

Sweden

2012

58°53′20.29″N

11° 6′31.94″E

Exposed

(8) oSW2

12 (10/2)

12 (10/2)

12 (10/2)

8 (8/0)

Anglesey South 1

Wales (UK)

2012

53°25′20.30″N

4°22′7.86″W

Sheltered

(9) oWA1

12 (8/4)

12 (8/4)

9 (6/3)

11 (8/3)

Anglesey South 2

Wales (UK)

2012

53°25′5.74″N

4°27′10.82″W

Exposed

(10) oWA2

12 (7/5)

9 (6/3)

11 (7/4)

7 (6/1)

Baiona

Spain

2012

42° 7′26.93″N

8°50′51.35″W

Sheltered

(16) oSP3

40 (28/12)

34 (24/10)

11 (11/0)

11 (11/0)

Rio de Moinhos

Portugal

2012

41°34′4.05″N

8°47′51.81″W

Sheltered

(17) oPT1

40 (30/10)

32 (24/8)

10 (10/0)

4 (4/0)

Total

      

209

166

123

84

L. fabalis

Sandgerdi

Icleand

2005

64° 2′0.27″N

22°44′11.82″W

NA

(3) fIC

12 (NA)

3 (NA)

5 (NA)

0

Seløyna 1

Norway

2012

60°38′13.62″N

4°47′33.72″E

Sheltered

(5) fNO1

26 (6/20)

12 (4/8)

5 (2/3)

2 (1/1)

Seløyna 2

Norway

2012

60°38′7.08″N

4°47′32.94″E

Exposed

(6) fNO2

12 (7/5)

8 (5/3)

7 (6/1)

5 (3/2)

Lökholmen 1

Sweden

2012

58°53′21.98″N

11° 6′38.61″E

Sheltered

(7) fSW1

12 (12/0)

11 (11/0)

8 (8/0)

8 (8/0)

Lökholmen 2

Sweden

2012

58°53′20.29″N

11° 6′31.94″E

Exposed

(8) fSW2

12 (12/0)

11 (11/0)

8 (8/0)

9 (9/0)

Anglesey South 1

Wales (UK)

2012

53°25′20.30″N

4°22′7.86″W

Sheltered

(9) fWA1

12 (12/0)

9 (9/0)

6 (6/0)

0

Anglesey South 2

Wales (UK)

2012

53°25′5.74″N

4°27′10.82″W

Exposed

(10) fWA2

12 (12/0)

9 (9/0)

5 (5/0)

4 (4/0)

Minard Castle

Ireland

2012

52° 7′12.00″N

10° 6′36.00″W

NA

(11) fIR

12 (12/0)

7 (7/0)

2 (2/0)

3 (3/0)

Wembury

England (UK)

2012

50°18′36.00″N

4° 5′60.00″W

NA

(12) fEN

12 (12/0)

7 (7/0)

2 (2/0)

2 (2/0)

Landunvez

France

2012

48°32′24.00″N

4°44′24.00″W

NA

(13) fFR

12 (12/0)

8 (8/0)

6 (6/0)

0

O Grove

Spain

2012

42°27′45.45″N

8°52′20.59″W

Zostera-Sheltered

(14) fSP1

25 (17/8)

21 (14/7)

9 (9/0)

4 (4/0)

Tirán

Spain

2012

42°15′48.01″N

8°45′11.63″W

Fucus-Intermediate

(15) fSP2

40 (31/9)

29 (22/7)

5 (4/1)

2 (1/1)

Póvoa de Varzim

Portugal

2012

41°23′7.24″N

8°45′88.30″W

Mastocarpus-Exposed

(18) fPT2

24 (12/12)

22 (10/12)

10 (7/3)

3 (3/0)

Total

      

223

157

78

42

Cabo do Mundoa

Portugal

2012

41°13′33.78″N

8°43′3.05″W

Sheltered

(19) PT3

30 (19/11)

21 (12/9)

24 (13/11)

7 (5/2)

  1. aLocality where hybrids between L. fabalis and L. obtusata were previously identified based on microsatellites (see [31])
  2. NA not available; Sampling locations code letters stand for the country: US USA, IC Iceland, NO Norway, SW Sweden, WA Wales, SP Spain, PT Portugal, IR Ireland, EN England, FR France