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Fig. 1 | BMC Evolutionary Biology

Fig. 1

From: Investigating cellular and molecular mechanisms of neurogenesis in Capitella teleta sheds light on the ancestor of Annelida

Fig. 1

Dividing NPCs undergo multiple rounds of division while maintaining apical contact. a, c Schematic showing stage 4 telotroch animals exposed to BrdU (red) for 2 h followed by a 3 h thymidine chase (pink arrow) and sea-water incubation (blue arrow) until 24 h a and 48 h c respectively before a 2 h EdU (cyan) pulse. b, b′, b”, d, d’, d”) Confocal micrographs of BrdU pulse-chase-wait-EdU experiments showing BrdU (red) and EdU (cyan) after chase for 24 h b–b″ and 48 h d–d”. Panels b, b′, b″, d, d’, d” represent ventral head views showing single BrdU+ (b, d), EdU+ (b′, d’) and EdU/BrdU dual-labeled cells (b″, d”). Closed arrowheads show EdU/BrdU dual-labeled cells at the apical surface of the anterior neuroectoderm. Dashed lines indicate the basal boundaries of the developing brain. The channel visualized is indicated on the lower left corner of each figure panel. ST4tt: Stage 4 telotroch, ST5l: Stage 5 late, ST6mid: stage 6 mid. Scale bar: 25 μm

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