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Fig. 7 | BMC Evolutionary Biology

Fig. 7

From: Distant hybrids of Heliocidaris crassispina (♀) and Strongylocentrotus intermedius (♂): identification and mtDNA heteroplasmy analysis

Fig. 7

Enzyme digestion of fragments from the parents of the Heliocidaris crassispina (♀) × Strongylocentrotus intermedius (♂) hybrids and from the pure offspring at each developmental stage. a: A 584 bp region was PCR amplified using total DNA as a template. The amplified fragment from the male parent (S. intermedius) contains an EcoR I-sensitive site. Thus, digestion of this PCR product produces two fragments (428 and 156 bp). However, the amplified fragment from the female parent (H. crassispina) is EcoR I-resistant. b: The fragments amplified from paternal mtDNA can be selectively digested and thus distinguished from the fragments amplified from maternal mtDNA (H. crassispina). Lane 1: PCR product resulting from maternal mtDNA amplification. This product could not be digested by the EcoR I restriction enzyme and was thus 584 bp long. Lane 2: PCR product resulting from paternal mtDNA amplification was successfully digested by the EcoR I restriction enzyme to produce 428 and 156 bp fragments. Lanes 3–5: the biparental mtDNA signal was detected at each stage. HC_F: H. crassispina Fujian population; SI_C: S. intermedius cultured population; HS hybrids: H. crassispina (♀) × S. intermedius (♂) hybrid

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